| Subject |
Expression of MAGE-A Genes and Soluble ICAM-1 in Egyptian Breast Cancer Patients: Possible Prognostic Impact |
| Authors |
El-Sayed L.H., Ghoneim H.M. , Fadali G., Saad A. , Hafez E.S and Shaaban S. |
| Code |
2010 -
vol. 31,
No. 1,
Page. 7
|
| Type |
Research Article
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| FILE #1 : 7-18==.pdf (377.0K), Down:3186, 2011-09-12 03:15:44 |
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Background: An increased understanding of the pathogenesis of breast
cancers is imperative to continuous efforts of innovative approaches for its
diagnosis and immunotherapy. Continuous search for potentially
prognostic biomarkers should be addressed. An interesting category of
tumor-associated antigens encoded by Melanoma-Associated Genes
(MAGE) and gene products involved in cancer progression like cell
adhesion molecules (CAMs) are innovative prognostic markers.
Aim: The present study was done to find out a possible prognostic impact
of MAGE-A and SICAM gene expression in Egyptian breast cancer.
Methods: This study included 51 females with breast tumors, 9 females
with benign fibroodenoma & 10 age matched control females MAGE-A
gene expression was done in extracts of both benign and malignant breast
tissues by conventional reverse transcript-xion-polymerase chain reaction
(RT-PCR). In addition, mRNA transcript-xs of soluble intercellular adhesion
molecule-1 (sICAM-1) were quantified in extracts of peripheral blood
mononuclear cells (PBMCs) by real time RT-PCR.
Results: Expression of MAGE-A1, 3 and 4 was detected in 43.1%, 54.9%
and 25.5% of malignant tumors respectively while undetectable expression
was shown by benign lesions. Interestingly, expression of these MAGE-A
alleles was much more pronounced in tumors the invasive lobular
carcinoma (ILC) histologic type, advanced stages, the ER positive
phenotype and lymph node metastasis. There was also a highly significant
increase in expression rate of sICAM-1 of breast cancer patients as
compared to those with benign lesions and healthy controls. Only tumors
at advanced stages and those with the ER negative phenotype showed
significantly increased expression of sICAM-1 as compared to their
corresponding partners.
Conclusion: It could be concluded, that sICAM-1 may play an important
role in the metastatic process and can be employed as an indicator of
metastasis in clinical settings.
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