ALEXANDRIA UNIVERSITY
MEDICAL TECHNOLOGY CENTER
MEDICAL RESEARCH INSTITUTE EXTENSION
EM UNIT
Basics &Principles
In
Practice of Electron Microscopy
June 23– 25, 2009
Organized by:
Prof.Dr. Metwally Aly Metwally Kotb
Head of Electron Microscope Unit
Medical Technology center
Alexandria University
During the fall of 2009, EM UNIT would like to invite all Transmission Electron Microscope (TEM) users and all other interested parties to our workshop, a review of new tools, a comprehensive approach to applications, support and training to make all techniques easier for everyone playing a role in the EM lab.
With these events, we like to open a dialog with the users of TEM, discuss their needs and requests, and demonstrate latest improvements, features and capabilities of TEM techniques.
We will provide an introduction to each topic, followed by interactive presentations and hands-on training at the TEM.
We are looking forward to meeting you.
If you have any questions concerning course subjects, directions or accommodations, please don't hesitate to contact us:
GENERAL PROGRAM
First Day
9:00 am – 9:30 am Registration
10:00 am – 11:30 am
Lecture: Electron Microscopy at a glance.
11:30 am – 12:00 pm coffee break
12:00 pm – 2:00 pm Practical session:
-Sample preparation for TEM.
-choosing the suitable fixative for your sample?
-Survey for different types of Epon and Araldite.
Second Day
10:00 am – 11:30 pm
Lecture: Applications OF Nano Technology in E.M
11:30 pm – 12:00 pm coffee break
12:00 pm – 01:00 pm
Lecture: Undifferential Tumor and Muscle disorders Using E.M
01:00 pm Practical session:
-Frozen sections.
-Continue Sample preparation for TEM.
-How to obtain glass knife by Knife Maker?
-How to make embedding media "Araldite/Epon"?
-How to examine samples under TEM.
-Film cuts processing.
-Preparation of viral samples & Blood sample to be examined under TEM.
-Hard tissues "Bone & Teeth" for TEM.
Third Day
9:00 am – 11:00 am
Lecture: Immuno histochemistry teqniques and their applications in E.M
-choice of fixative & perfect concentration
-frozen sections vs. embedded tissue
11:00 am – 12:00 pm
Lecture: Safety in the TEM Lab
12:00 pm – 12:30 pm coffee break
12:30 pm Practical session:
-Continue Sample preparation for TEM.
-The art of Ultramicrotomy.
-How to make capsules?
-Cutting the capsules through ultra microtome into semi thin and ultra thin sections.
-Discussion and Certificates
Total fees:
Egyptian Universities Staff Members: 450L.E.
Egyptian Universities students: 250 L.E
Others: 900 L.E.
Tel: (203) 42 66 213/17/18
Fax: (203) 42 66 214
Add.: 71 Vector Emanuel St., Smouha, Alexandria
E-mail: mtcalex@link.net