ELECTRON MICROSCOPY
(Three workshops during the year
2008)
Organized by: Prof. Dr. Metwally Kotb
NB. Enrollment limited to
15 participants
Aim of the workshop:
Providing the participants with an
overview about the transmission E.M. to be familiar with the E.M. system and its
accessories such as: knife maker, ultra microtome, specimens carrying grids, the
E.M. holder and the specimen holder, preparation of the different biological
materials, chemicals needed, hazards produced by the E. M. and the precautions
required to obtain good specimens; in addition to, the developing, fixation, and
handling the micrographs.
Participants:
Participants are expected to be qualified personnel who hold a
university degree from one of the following faculties:
ｷ
Faculty of Medicine
ｷ
Faculty of Dentistry
ｷ
Faculty of Pharmacy
ｷ
Faculty of Science
ｷ
Faculty of Agriculture
ｷ
Faculty of Veterinary
E.
M. workshop Program:
Day 1:
10:30 ・11:30 Electron Microscopy: Principles and applications
11:30 ・12:00 Break
12:00 ・03:00 Visiting the electron microscopy laboratory:
Processing and preparation of biological specimens for EM study
Day 2:
10:00 ・12:00 Special techniques in EM processing:
1- Blood
2- Bone
12:00 ・12:30 Break
12:30
・03:00 Special techniques in EM processing (continuing):
3- Bacteria and Viruses
4- Ultra-structure histochemistry
Day 3:
10:00 ・12:00 Cell ultra-structure (normal and abnormal cell
structure)
12:00 ・12:30 Break
12:30 ・03:00 Processing and preparation of biological specimens
(continuing):
1- Glass knifes
preparation for the microtome
2- Preparation
of specimen solid capsules and grids
3- Grids
examination and photomicrography
Day 4:
10:00 ・11:30 Imaging, developing and printing the EM
pictures
11:30 ・12:00 Break
12:00 ・03:00 Application of electron microscopy in disease
diagnosis (renal, skeletal muscles, tumors,・etc.)
Interpretation of EM
micrographs and open discussion