Immunology
(Two workshops during the year
2008)
Organized by: Prof. Dr. Laila H. El-Sayed
NB. Enrollment limited to 15
participants
(I)
Basic Diagnostic Immunology
Day
1:
Lectures:
1- Introduction to
Immunology
2- Organs and Cells of
the immune system
Practical:
1- Isolation of
lymphocytes from human peripheral blood. Enumeration and analysis of T&B
cells and other lymphoid cell populations
2- Dissection of a
mouse for identification of different lymphoid organs (Thymus, spleen and lymph
nodes)
Day
2:
Lectures:
1- Antigens and
immunogens
2- Immunoglobulins:
structure and function
3- An overview of
antigen antibody interaction: Methods of detection
Practical:
1- ELISA
2- Immunoprecipitation:
Turbidimetry, Nephlometry, Immunodiffusion and Immune complex
determination
Day
3:
Lectures:
1- Effector mechanism
of nonspecific immune responses
2- HLA: clinical
association with diseases
Practical:
1- Phagocytosis:
experimental verification and immunologic application
2- Tissue typing:
serological and PCR assays
Day
4:
Lectures:
1- Cytokines, adhesion
molecules, and other mediators of the Immune systems
2- Effector mechanisms
of specific immune response
3- PCR: Technique and
applications
Practical:
1- Immune conjugation
reactions: Chemiluminescence, ELISA and RIA and their diagnostic
applications
Day
5:
Lectures:
1- Immunity to
infectious diseases
2- Value of
immunohistochemistry in diagnostic pathology
Practical:
1- Immune conjugation:
Immunofluoresence, Immunohistochmistry and their application in diagnostic
pathology and autoimmune diseases
Day
6:
Lectures:
1- Complement system in
diseases
2- Hypersensitivity
reaction
Practical:
1- SDS and western
blot
2- Haemmagglutination
(II)
Molecular Immunology: Theory, practice and application
Day 1:
Lectures:
1- Introduction to
Molecular Immunology (Antibodies, Cell receptors, Cytokines, etc)
Practical:
1- Application of
Molecular Immunology in Diagnosis
Day 2:
Lectures:
1- Antigens and their
immunologic recognition (Application in microbial infections)
Practical:
1- Antigen separation
and purification (Chromatography, preparative electrophoresis, etc)
Day 3:
Lectures:
1- Recombinant DNA
technology (the book of life: from gene to protein and from protein to
machine)
Practical:
1- Isolation and
purification of DNA, RNA, and total soluble proteins
Day 4:
Lectures:
1- Gene expression
analysis: gene quantification technology
Practical:
1- Gene
cloning
2- Polymerase Chain
Reaction (PCR): theory, optimization, and applications
Day 5:
Lectures:
1- PCR applications in
molecular immunology
Practical:
1- Screening of
recombinant proteins
2- Extraction and
purification of recombinant proteins by SDS-PAGE
3- Identification of
recombinant proteins by Western Blotting
Day 6:
Lectures:
1- Recent advances in
DNA vaccinology
2- DNA fingerprinting
technology: techniques and applications
Practical:
1- In vitro
transcript-xion of genes in prokaryotic expression vectors
2- Human DNA
fingerprinting: case studies