Prof. Dr. Mohamed Samy Afifi M.D, Ph.D (Boston University, USA) Head, Immunology Department Medical Research Institute Alexandria University
Hands on: BASICS Techniques In Cell Culture
3-Day Cell Culture
The purpose of the Cell Culture Workshop is to provide a solid understanding of basic cell culture techniques through classroom and laboratory work. Attendants will gain significant experience in the manipulation of cells through culture. Through integrated learning techniques, utilizing hands-on training, and lectures, attendants will learn the biological basis and relevance of the process of culturing cells. This will allow interested attendants to translate the techniques and information learned in this workshop into their own laboratories This workshop provides prerequisite training for the culture of numerous cell types.
Who should attend?
Personnel in the fields of Immunology, Virology, Microbiology, as well as all laboratory personnel dealing with in-vitro fertilizations, and tissue typing. Also, pharmaceutical laboratories personnel and members of the Egyptian Society of Immunology.
Course Programme
09:30 AM – 11:30 AM Lectures 11:30 AM – 12:00 PM Break 12:00 PM – 04:00 PM Practical work
A certificate will be awarded upon completion of the course
Topics Covered
1)Introduction to cell and tissue culture. 2)Serum-containing and Serum-free culture media. 3)Trypsinization. 4)Cell counting, viability and growth curves. 5)Growth of adherent and non- adherent cells. 6)Suspension culture differentiation and attachment. 7)Adaptation to suspension culture. 8)Adaptation to suspension serum-free media. 9)Cryo preservation and cryo thawing. 10)Cell cloning, primary culture and the cell cycle.
Speakers
Teaching Staff of the Immunology department, Medical Research Institute, Alexandria University.
Registration Fees
550 LE for Egyptian university staff members 1000 LE for others Accommodation is available upon prior request of at least one month before the course start date: 100 LE for Single room 150 LE for Double room