Microwave processing technique
for electron microscopy
6 - 9 of February 2012
INTRODUCTION
Microwave-accelerated
sample preparation of biological specimens is a field that continues to
grow rapidly as evidenced by the number of innovated articles written
each year. The reason for this increase in the microwave literature is
simple. Microwave accelerated procedure speed up reaction processes and
save time. In short, microwave-accelerated technique can be used to
improve the efficiency of a variety of histopathology laboratory
procedures such as fixation, decalcification, processing of specimen for
paraffin or resin embedding and staining.
WORKSHOP OBJECTIVES
The
intended audience is students and researchers with expertise interest
in their field of research using electron microscope. This workshop aims
to introduce the use of home microwave oven to accelerate the
processing technique in electron microscopy lab. In addition of giving
an intensive training to all equipment related to sample preparation
including animal perfusion, ultramicrotomy and electron microscope.
Topics also cover the basic parameters, which influence the image, and
resolution.
WORKSHOP OUTLINE
In addition to practical sessions, this workshop includes the following lectures:
* Fundamentals of electron microscopes construction.
* Microwave processing technique for electron microscopy.
* Artifacts confuse interpretations of TEM
* Ultra structure of normal cell organelles
PARTICIPANT
All members interested in:
* a) Biology
* b) Medical Research
* c) Pharmaceutical science
* d) Physics
* e) Microbiology
* f) Agriculture Science
* g) Environmental studies
* i) Veterinary Medicine
WORKSHOP FEES
For Egyptian: 600 L.E.
For others: 1000 L.E.
ACCOMMODATION
Is available upon prior request of at least one week before the course start date:
100 L.E. for Single room/day
150 L.E. for Double room/day
Workshop program
ORGANIZERS
Prof. Dr. Gamal El-Dein El-Sawaf
Dean of Medical Research Institute, Alexandria University.
Dr. Nabil Elboraie Elboceiry
Biophysics Dept., Medical Research Institute, Alexandria University.
SPEAKERS
Prof. Dr. Jaylan Fadaly, Pathology Dept., Medical Research Institute, Alexandria University.
Prof. Dr. Sohair Elkhouly, Biophysics Dept., Medical Research Institute, Alexandria University.
Dr. Nabil Elboraie Elboceiry, Biophysics Dept., Medical Research Institute Alexandria University.
PRACTICAL WORK
Dr. Nabil Elboraie Elboceiry, Biophysics Dept., Medical Research Institute,
Alexandria University
Vet. El-Sayeda Zeinab Medical Technology Center, Alexandria University
REGISTRATION
For further enquires and registration, please contact:
Mrs. Mai Kotb Mob: 01063436912
Phone: (203) 42 66 213 / 17 / 18
Fax: (203) 42 66 214 E-mail: medrinal@hotmail.com
Day-Time
| 9.00 – 11.00 |
| 11.30-12.30 | 12.30 – 3.30 Practical session |
Sunday 6/2/2012 | -Opening ceremony Microwave processing technique for electron microscopy. | Coffee Break | Animal perfusion, selection of different samples. | Microwave sample processing. |
Monday 7/2/2012 | Artifacts confuse interpretations of TEM micrographs. | Coffee Break | Fundamentals of electron microscopes constructions | Staining. |
Tuesday 8/2/2012 | Ultra structure of normal cell organelles | Coffee Break | Investigation on TEM. | Investigation on TEM. |
Wednesday 9/2/2012
| More TEM practice: | Coffee Break | General discussion | -Photography. -Closing ceremony: The award of certificates by Prof. Dr. Gamal El-Dein El-Sawaf |