Workshop of electron microscopy unit"19th - 22nd of July 2009"Medical Research Institute, Alexandria University
INTRODUCTION:
The quality of work and skill performance requires knowledge about different operation modes of the electron microscope and proper preparation of samples. It is in this regard that the electron microscope should properly aligned, astigmatism free as well as considered different operation mode. The microstructure at highest levels of resolution is the goal of this workshop. Also an interpretation of pathogenic changes compared with poorly fixed samples should be taken into consideration.
Of particular interest in this workshop is to prepare many samples to study its internal structure. Preparation includes different tissues of normal mice and bacteria.
Course Objective:
The intended audience is PhD students and researchers with expertise/interest in their field of research using electron microscope. Therefore this workshop aims to train participants to be familiar with the basic parameters, which influence the image. Topics covered include the illumination system, electron lenses and their aberrations, image formation and resolution, the vacuum system and contamination. This course also aims to train participants to be familiar with the basic parameters, which affect sample preparations.
The course outline:
In addition to practical sessions, this workshop includes the following lectures:
Basic concepts of electron microscopy.
Electron microscopy and cell biology.
Sample preparation techniques.
Deception by technical artifacts during interpretation of TEM micrographs.
Ultrastructure of bacteria.
Participant:
All members interested in:
a) Microbiology b) Medical Research
c) Pharmaceutical science d) Physics
e) Biochemistry f) Agriculture Science
g) Environmental studies h) Botany
Course Fees: 250 L.E.
Workshop program
Registration:
Send e-mail to biomedical.electronics@yahoo.com for more information call Prof. Dr. Moustafa M. Mohamed, Department of Biophysics,
Tel No: 03 4261073
Registration deadline: 1. July - limited number of participants!
Organizers:
Prof. Dr. M.M. Mohamed, Head of Biophysics Department, Medical Research Institute, Alexandria University.
Dr. Nabil Elboraie Elbossiry, Biophysics Department, Medical Research Institute, Alexandria University.
Speakers:
- Prof. Dr. Shefa Mohammed Fakhr Eldin Elsawi Prof. of Histology & Cell Biology, Faculty of Medicine, Alexandria University.(Electron microscopy and cell biology).
- Dr. Ghada Mourad Ass. Prof. Histology & CellBiology; Faculty of Medicine, Alexandria University, Egypt. (Deception by technical artifacts during interpretation of TEM micrographs).
- Dr. Abeer Ghazal Ass. Prof. of Microbiology, Medical Research Institute, Alexandria University, Egypt. (Ultrastructure of Bacteria)
- Dr. Nabil Elboraie, Medical Research Institute, Alexandria University, Egypt (Basic concepts of electron microscopy and sample preparation techniques).
Topics:
|
Time
Day |
9 - 10 |
10 – 10.30 |
10.30 – 11.30 |
11.30 – 3
Practical session |
|
Sunday
19 / 7 / 2009 |
Deception by technical artifacts during interpretation of TEM micrographs |
Coffee break |
Sample preparation techniques.
|
Pre-fixation, Post-fixation, dehydration and infiltration of different samples as mice tissues and bacteria. |
|
Monday
20 / 7 / 2009 |
Basic concepts of electron microscopy. |
Basic concepts of electron microscopy. |
Embedding and training on sectioning of some already prepared samples. |
|
Tuesday
21 / 7 / 2009 |
Electron microscopy and cell biology. |
Ultrastructure of Bacteria |
Sectioning of samples which, were prepared in the workshop. |
|
Wednesday
22 / 7 / 2009 |
Investigation on TEM |
Investigation on TEM |
Certificates distribution |