MICROWAVE PROCESSING TECHNIQUE
FOR ELECTRON MICROSCOPY
An Electron Microscope Workshop
3 - 5 of December 2012
Introduction
Microwave-accelerated
sample preparation of biological specimens is a field that continues to
grow rapidly as evidenced by the number of innovated articles written
each year. The reason for this increase in the microwave literature is
simple. Microwave accelerated procedure speed up reaction processes and
save time. In short, microwave-accelerated technique can be used to
improve the efficiency of a variety of histopathology laboratory
procedures such as fixation, decalcification, processing of specimen for
paraffin or resin embedding and staining.
WORKSHOP OBJECTIVES
The
intended audience is students and researchers with expertise interest
in their field of research using electron microscope. This workshop aims
to introduce the use of home microwave oven to accelerate the
processing technique in electron microscopy lab. In addition of giving
an intensive training to all equipment related to sample preparation
including animal perfusion, ultramicrotomy and electron microscope.
Topics also cover the basic parameters, which influence the image, and
resolution.
WORKSHOP OUTLINE
In addition to practical sessions, this workshop includes the following lectures:
1. Fundamentals of electron microscopes construction.
2. Sample preparation techniques
3. Microwave processing technique for electron microscopy.
4. Artifacts confuse interpretations of TEM
PARTICIPANT
All members interested in:
a) Biology
b) Medical Research
c) Pharmaceutical science
d) Physics
e) Microbiology
f) Agriculture Science
g) Environmental studies
i) Veterinary Medicine
WORKSHOP FEES
For Egyptian: 850 L.E.
For others: 1000 L.E.
ACCOMMODATION
Is available upon prior request of at least one week before the course start date:
100 L.E. for Single room/day
150 L.E. for Double room/day
Workshop program
Organizers:
Prof. Dr. Mohamed Abdel Mohsen, Director Manager of Medical technology center Alexandria University, Alexandria.
Dr. Nabil Elboraie Elboceiry, Biophysics Department, Medical Research Institute, Alexandria University.
Speakers:
Prof Dr. Geylan Fadaly, Pathology Department, Medical Research Institute, Alexandria University.
Prof Dr. Sohair El-Khouly, Biophysics Department, Medical Research Institute, Alexandria University.
Dr. Nabil Elboraie Elboceiry, Biophysics Department, Medical Research Institute, Alexandria University, Egypt.
PRACTICAL WORK
Dr. Nabil Elboraie Elboceiry, Biophysics Department, Medical Research Institute, Alexandria University, Egypt.
Vet. El-Sayeda Zeinab, Medical Technology Center, Alexandria University
Pharmacist Manar Mokhtar, Medical Technology Center, Alexandria University
REGISTRATION
For further enquires and registration, please contact:
Mrs. Mai Kotb Mob: 01063436912
Phone: (203) 42 66 213 / 17 / 18
Fax: (203) 42 66 214
E-mail: medrinal@hotmail.comTopics :-
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Time
Day
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9-10
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10 – 10.30
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10.30 – 11.30
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11.30 – 3
Practical session
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Monday
3 / 12 / 2012
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Opening ceremony Microwave
processing technique for electron microscopy.
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Coffee break
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Animal perfusion, selection of
different samples.
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Microwave sample processing
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Tuesday
4 /12 / 2012
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Artifacts confuse interpretations
of TEM micrographs
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Fundamentals of electron microscopes
constructions
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Sectioning of some the prepared samples
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Wednesday
5 / 12 / 2012
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Ultra
structure and function of normal cell organelles.
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Investigation on TEM
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Photography. -Closing ceremony: The award
of certificates by Prof. Dr. Mohamed Abdel Mohsen
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