University of Alexandria
Medical Research Institute
Medical Technology Center
ELECTRON MICROSCOPE UNIT
ULTRASTRUCTURE
OF PLANT CELL AND BACTERIA
BY
TRANSMISSION ELECTRON MICROSCOPE
Organized By:
Dr. Nabil Elboraie Elboceiry
Biophysics Department
Medical Research Institute
Alexandria University
09 – 10 September 2012
INTRODUCTION
The goal of this workshop is to prepare plant tissue and bacteria for investigation by transmission electron microscope. In this workshop, plant cells contain a lot of air gases, this will affect infiltration process. The use of vacuum will facilitate the process. Bacteria will be obtained in solution form. Each step of preparation requires centrifugation. Skill performance requires knowledge about different steps of sample preparation. This can be achieved when you carefully adapt with all precaution during preparation of samples. In addition of bacterial sample preparation the basic technique of all sample preparation for studying by transmission electron microscope will also be covered.
OBJECTIVES
The intended audience is students and researchers with expertise interest in their field of research using electron microscope. The main object of this workshop is to know the proper and simple method to prepare your samples in your lab. In addition of giving an intensive training to all equipment related to sample preparation including, ultramicrotomy and electron microscope. Topics also cover the basic parameters, which influence the image, and resolution.
OUTLINE
In addition to practical sessions, this workshop includes the following lectures:
• Fundamentals of electron microscopes construction.
• Sample preparation for electron microscopy.
• Artifacts confuse interpretations of TEM
• Ultra structure of bacteria, and its organelles.
PARTICIPANT
All members interested in:
• Pharmaceutical science
• Microbiology
• Agriculture Science
• Medical Research
• Environmental studies
• Veterinary Medicine
WORKSHOP PROGRAM
Sunday 09-09-2012:
09:00 AM – 10:00 AM
Opening ceremony
Sample preparation for electron microscopy
10:00 AM – 10:30 AM
Coffee break
10:30 AM – 11:30 AM
Fundamentals of electron microscopes constructions
11:30 AM – 02:30 PM
Plant and bacteria samples preparation
Monday 10-09-2012:
09:00 AM – 10:00 AM
Artifacts confuse interpretations of TEM micrographs
10:00 AM – 10:30 AM
Coffee break
10:30 AM – 11:30 AM
Ultrastructure of bacteria and plant
11:30 AM – 02:30 PM
*Ultamicrotomesectioning. Staining. *Investigation on TEM.
*Photography.
ORGANIZERS
Dr. Mohamed Abdel-Mohsen
Director Manager, Medical Technology Center, Alexandria University
Dr. Nabil Elboraie Elboceiry
Biophysics Dept., Medical Research Institute, Alexandria University
SPEAKERS
Prof. Sohair Elkhouly
Biophysics Dept., Medical Research Institute, Alexandria University.
Dr. Nabil Elboraie Elboceiry
Biophysics Dept., Medical Research Institute Alexandria University
Dr. Sherine Shawky
Microbiology Dept., Medical Research Institute Alexandria University
Pharmacist. Manar Mokhtar
Electron Microscope Unit. Medical Technology Center
PRACTICAL WORK
Dr. Nabil Elboraie Elboceiry
Biophysics Dept., Medical Research Institute Alexandria University
REGISTRATION FEES
For Egyptian: 400 L.E.
For others: 800 L.E.
For Non Egyptian: 800 $
ACCOMMODATION
Is available upon prior request of at least one week before the course start date:
100 LE for Single room/day
150 LE for Double room/day
Non-Egyptian:
35$ for Single room/day
50$ for Double room/day
FOR FURTHER ENQUIRIES AND REGISTRATION, PLEASE CONTACT:
Mrs. Mai Kotb Mob: 01063436912
Add: 71 Victor Emanuel St., 21615,
Al-Azhar square, Smouha,
Alexandria, Egypt
Phone : (203) 42 66 213 / 17 / 18
Fax : (203) 42 66 214
E-mail : medrinal@hotmail.com