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Ultrastructure of plant cell and bacteria by Transmission Electron Microscope
Electron microscopy workshop
16 - 17 of July 2012
The goal of this workshop is to prepare plant tissue and bacteria for investigation by transmission electron microscope. In this workshop, plant cells contain a lot of air gases, this will affect infiltration process. The use of vacuum will facilitate the process. Bacteria will be obtained in solution form. Each step of preparation requires centrifugation. Skill performance requires knowledge about different steps of sample preparation. This can be achieved when you carefully adapt with all precaution during preparation of samples. In addition of bacterial sample preparation the basic technique of all sample preparation for studying by transmission electron microscope will also be covered.
WORKSHOP OBJECTIVES
The intended audience is students and researchers with expertise interest in their field of research using electron microscope. The main object of this workshop is to know the proper and simple method to prepare your samples in your lab. In addition of giving an intensive training to all equipment related to sample preparation including, ultramicrotomy and electron microscope. Topics also cover the basic parameters, which influence the image, and resolution.
WORKSHOP OUTLINE
In addition to practical sessions, this workshop includes the following lectures:
• Fundamentals of electron microscopes construction.
• Sample preparation for electron microscopy.
• Artifacts confuse interpretations of TEM
• Ultra structure of bacteria, and its organelles.
PARTICIPANT
All members interested in:
• a) Pharmaceutical science
• b) Microbiology
• c) Agriculture Science
• d) Medical Research
• e) Environmental studies
• f) Veterinary Medicine
WORKSHOP FEES
For Egyptian: 400 L.E.
For others: 800 L.E.
ACCOMMODATION
Is available upon prior request of at least one week before the course start date:
100 L.E. for Single room/day
150 L.E. for Double room/day
ORGANIZERS
Prof. Dr. Mohamed Abdelmohsen
Director of Medical Technology center, Medical Research Institute, Alexandria University.
Dr. Nabil Elboraie Elboceiry
Biophysics Dept., Medical Research Institute, Alexandria University.
SPEAKERS
Prof. Dr. Sohair Elkhouly, Biophysics Dept., Medical Research Institute, Alexandria University.
Dr. Dalia Elsayed, Microbiology Dept., Medical Research Institute Alexandria University
Dr. Nabil Elboraie Elboceiry, Biophysics Dept., Medical Research Institute Alexandria University
PRACTICAL WORK
Dr. Nabil Elboraie Elboceiry, Biophysics Dept., Medical Research Institute, Alexandria University
REGISTRATION
For further enquires and registration, please contact:
Mrs. Mai Kotb Mob: 01063436912
Phone: (203) 42 66 213 / 17 / 18
Fax: (203) 42 66 214
E-mail: medrinal@hotmail.com
Topics:
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Time
Day
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9.00 – 10.00
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10.0-10.30
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10.30-11.30
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11.30 – 2.30
Practical session
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Monday
16/7/2012
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Opening ceremony
Sample
preparation for electron microscopy
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Coffee
break
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Fundamentals
of electron microscopes constructions
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Plant and bacteria samples preparation.
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Tuesday
17/7/2012
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Artifacts
confuse interpretations of TEM micrographs.
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Ultrastructure
of bacteria and plant
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Ultamicrotome sectioning.
Staining. Investigation on TEM.
Photography.
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