Microwave processing technique for electron microscopy

6 - 9 of February 2012

INTRODUCTION

Microwave-accelerated sample preparation of biological specimens is a field that continues to grow rapidly as evidenced by the number of innovated articles written each year. The reason for this increase in the microwave literature is simple. Microwave accelerated procedure speed up reaction processes and save time. In short, microwave-accelerated technique can be used to improve the efficiency of a variety of histopathology laboratory procedures such as fixation, decalcification, processing of specimen for paraffin or resin embedding and staining.

WORKSHOP OBJECTIVES

The intended audience is students and researchers with expertise interest in their field of research using electron microscope. This workshop aims to introduce the use of home microwave oven to accelerate the processing technique in electron microscopy lab. In addition of giving an intensive training to all equipment related to sample preparation including animal perfusion, ultramicrotomy and electron microscope. Topics also cover the basic parameters, which influence the image, and resolution.

WORKSHOP OUTLINE

In addition to practical sessions, this workshop includes the following lectures:

* Fundamentals of electron microscopes construction.

* Microwave processing technique for electron microscopy.

* Artifacts confuse interpretations of TEM

* Ultra structure of normal cell organelles

PARTICIPANT

All members interested in:

* a) Biology

* b) Medical Research

* c) Pharmaceutical science

* d) Physics

* e) Microbiology

* f) Agriculture Science

* g) Environmental studies

* i) Veterinary Medicine

WORKSHOP FEES

For Egyptian: 600 L.E.

For others: 1000 L.E.

ACCOMMODATION

Is available upon prior request of at least one week before the course start date:

100 L.E. for Single room/day

150 L.E. for Double room/day

Workshop program

ORGANIZERS

Prof. Dr. Gamal El-Dein El-Sawaf

Dean of Medical Research Institute, Alexandria University.

Dr. Nabil Elboraie Elboceiry

Biophysics Dept., Medical Research Institute, Alexandria University.

SPEAKERS

Prof. Dr. Jaylan Fadaly, Pathology Dept., Medical Research Institute, Alexandria University.

Prof. Dr. Sohair Elkhouly, Biophysics Dept., Medical Research Institute, Alexandria University.

Dr. Nabil Elboraie Elboceiry, Biophysics Dept., Medical Research Institute Alexandria University.

PRACTICAL WORK

Dr. Nabil Elboraie Elboceiry, Biophysics Dept., Medical Research Institute,Alexandria University

Vet. El-Sayeda Zeinab Medical Technology Center, Alexandria University

REGISTRATION

For further enquires and registration, please contact:

Mrs. Mai Kotb Mob: 01063436912

Phone: (203) 42 66 213 / 17 / 18

Fax: (203) 42 66 214 E-mail: medrinal@hotmail.com

Day-Time	9.00 – 11.00		11.30-12.30	12.30 – 3.30 Practical session
Sunday 6/2/2012	-Opening ceremony Microwave processing technique for electron microscopy.	Coffee Break	Animal perfusion, selection of different samples.	Microwave sample processing.
Monday 7/2/2012	Artifacts confuse interpretations of TEM micrographs.	Coffee Break	Fundamentals of electron microscopes constructions	Staining.
Tuesday 8/2/2012	Ultra structure of normal cell organelles	Coffee Break	Investigation on TEM.	Investigation on TEM.
Wednesday 9/2/2012	More TEM practice:	Coffee Break	General discussion	-Photography. -Closing ceremony: The award of certificates by Prof. Dr. Gamal El-Dein El-Sawaf