Saturday 11-2-2006

DNA structure and function

Practical hints for extraction of DNA

Practical session (DNA extraction)

Sunday 12-2-2006

RNA structure and function

Practical hints for extraction of RNA

Practical session (RNA extraction)

Monday 13-2-2006

Protein extraction and characterization

Practical session I (protein extraction)

Tuesday 14-2-2006

Practical session II (SDS-PAGE)

Practical session III (staining, destaining, and visualization)

Saturday 4-3-2006

Principles of PCR

Detection of polymorphism by PCR

Practical session

Sunday 5-3-2006

RT-PCR

Practical session

Monday 6-3-2006

Other types of PCRs

Tuesday 7-3-2006

Principles of Western plot analysis

Practical session (SDS-PAGE, blotting)

Wednesday 8-3-2006

Blocking membrane

Hybridization with Abs

Thursday 9-3-2006

Visualization

Saturday 6-5-2006

Importance of subcloning

Cloning vectors and restriction endonucleases

Practical session (DNA digestion)

Sunday 7-5-2006

Introductory lecture for the Practical session Practical session (DNA phosphorylation and Ligation)

Monday 8-5-2006

Bacterial transformation

Practical session (preparation of competent cells and transformation)

Tuesday 9-5-2006

Verification of cloning

Practical session (DNA Mini. Prep. and digestion)

Wednesday 10-5-2006

Principles of Southern plot analysis

Practical session (DNA labeling and gel electrophoresis)

Thursday 11-5-2006

Practical session (Blotting, hybridization, and visualization)