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Course  Basics & Principles in Practice of Electron Microscopy
Period   2008. 12. 14. ~ 2008. 12. 16.

Alexandria University

Medical Technology Center for Research and Services

Medical Research Institute extension

 

EM UNIT


Basics & Principles in Practice of Electron Microscopy

 

December 14 – 16 and 18 - 20, 2008

 

 

 

 

Organized by:

The Medical Research Institute Staff Members

Electron microscopy unit

 

 

Tel:       203 42 66 213/17/18

Fax:      203 42 66 214

Add.:     71 Vector Emanuel   

             St., Smouha, Alexandria

E-mail: mtcalex@link.net

 

 

During the fall of 2008, EM UNIT would like to invite all Transmission Electron Microscope (TEM) users and all other interested parties to our workshop, a review of new tools, a comprehensive approach to applications, support and training to make life easier for everyone in the EM lab.

With these events, we like to open a dialog with the users of TEM, discuss their needs and requests, and demonstrate latest improvements, features and capabilities of TEM techniques.

We will provide an introduction to each topic, followed by interactive presentations and hands-on training at the TEM (if available).

 

We are looking forward to meeting you.

If you have any questions concerning course subjects, directions or accommodations, please don't hesitate to contact us:

mtcalex@link.net

 

GENERAL PROGRAM

 

First Day

9am – 9.30 am Registration

 

10 am – 11.30 am

 Lecture: Electron Microscopy at a glance.

 

11.30 am – 12 pm coffee brake

 

12 pm – 2 pm  Practical session:

-Sample preparation for TEM.

-How to choose a suitable fixative for your sample?

-A survey for different types of Epon and Araldite.

 

Second Day

9.30 am – 11 am

 Lecture: Immuno – electron microscopy

-          choice of fixative & perfect concentration

-          frozen sections vs embedded tissue

 

11am – 12 pm  coffee brake

 

12 pm – 2 pm

 Practical session:

-Frozen sections.

-Continue Sample preparation for TEM.

-How to obtain glass knife by Knife Maker?

-How to make embedding media "Araldite/Epon"?

-How to examine samples under TEM.

-Film cuts processing.

-Preparation of viral samples & Blood sample to be examined under TEM.

-Hard tissues "Bone & Teeth"  for TEM.

 

Third Day

9.30 am – 10.30 am

 Lecture: Safety in the TEM Lab.

 

10.30 am – 11.30 am

 Lecture:

The applications on the Electron Microscope.

 

11.30 am – 12 pm coffee brake

 

12 pm – 2 pm Practical session:

 

-Continue Sample preparation for TEM.

-The art of Ultramicrotomy.

-How to make capsules?

-Cutting the capsules through ultra microtome into semi thin and ultra thin Sections.

 

-Discussion and Certificates.


Total fees:


Egyptian Universities staff Members :    450 L.E

Others : 900 L.E

 

 



 

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