University of Alexandria

Medical Research Institute

Medical Technology Center

Immunology unit

BASICS Techniques

In

Cell Culture

Organized By

Prof. Dr. Mohamed Samy Afifi 

M.D, Ph.D

Boston University, USA

Head, Immunology Department

Medical Research Institute

Alexandria University

Available In Two Periods:

23-25 November 2008

27-29 November 2008

3-Day Cell Culture

   
The purpose of the Cell Culture Workshop is to provide a solid understanding of basic cell culture techniques through classroom and laboratory work. Attendants will gain significant experience in the manipulation of cells through culture. Through integrated learning techniques, utilizing hands-on training, and lectures, attendants will learn the biological basis and relevance of the process of culturing cells. This will allow interested attendants to translate the techniques and information learned in this workshop into their own laboratories

This workshop provides prerequisite training for the culture of numerous cell types.

Who should attend?

Personnel in the fields of Immunology, Virology, Microbiology, as well as all laboratory personnel dealing with in-vitro fertilizations, and tissue typing. Also, pharmaceutical laboratories personnel and members of the Egyptian Society of Immunology.

Course Programme

09:30 AM – 11:30 AM  Lectures

11:30 AM – 12:00 PM  Break

12:00 PM – 04:00 PM  Practical work

A certificate will be awarded upon completion of the course

 Topics Covered

1)     Introduction to cell and tissue culture.

2)     Serum-containing and Serum-free culture media.

3)     Trypsinization.

4)     Cell counting, viability and growth curves.

5)     Growth of adherent  and non- adherent  cells.

6)     Suspension culture differentiation and attachment.

7)     Adaptation to suspension culture.

8)     Adaptation to suspension serum-free media.

9)     Cry preservation and cryo thawing.

10) Cell cloning, primary culture and the cell cycle.

Speakers

Teaching Staff of the Immunology department, Medical Research Institute, Alexandria University.

Registration Fees

400 LE for Egyptian university stuff members

800 LE for non Egyptian university stuff members

Accommodation for outstation participants can be arranged upon prior request of at least one month before the course:

75 LE for Single room

100 LE for Double room

For further enquiries, please contact

Dr. El-Hassan Mostafa

Rosaline Mounir

Phone:  203 42 66213 / 17 / 18

FAX:      203 42 66214

E-mail: mtcalex@link.net